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  • Muhammad Zain Rasheed

FC in Genetics: Current Status and Future Directions

Flow Cytometry (FC) is a laboratory technique that is used to measure the physical and chemical characteristics of cells. In genetics, FC is used to analyze cells and to isolate specific cell populations based on their surface markers, DNA content, and other characteristics. The current status of FC in genetics is that it is widely used in the field of genetic research, particularly in the study of hematopoietic stem cells, T-cell development, and the analysis of chromosomal abnormalities. FC is also used in the diagnosis and monitoring of genetic disorders such as Sickle Cell Anemia and Thalassemia. In recent years, there has been increasing interest in using FC for the isolation of specific cell populations for genetic engineering and gene therapy applications. FC can be used to isolate cells with specific genetic modifications, such as induced pluripotent stem cells (iPSCs) and T-cells that have been genetically modified to express chimeric antigen receptors (CARs) for cancer therapy. Future directions for FC in genetics include the development of more sensitive and specific assays for the detection and isolation of cells with specific genetic modifications. Additionally, researchers are exploring the use of FC in combination with other technologies, such as CRISPR-Cas9 gene editing and single-cell sequencing, to provide a more comprehensive understanding of the genetic changes in cells and their role in disease development. Additionally, FC is being used in combination with machine learning algorithms to improve the detection and characterization of cells with specific genetic modifications, which can help to improve the precision and efficiency of genetic engineering and gene therapy applications.

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